alexa fluor 647 conjugated ha tag 6e2 mouse mab  (Cell Signaling Technology Inc)

Journal: bioRxiv

Article Title: CRISPR screen identifies CNIH1 as a selective driver of GPCR export

doi: 10.1101/2025.10.27.684930

Figure Lengend Snippet: (a) Confocal microscopy images of HeLa cells expressing CNIH1 (top) or CNIH4 (bottom) fused N-terminally to an HA tag. Cells were fixed and immunostained with anti-HA (magenta) and anti-Giantin (cyan) antibodies. Merged images show localization of CNIH1 in the Golgi area, which is less pronounced for CNIH4. Images are confocal slices. Scale bar 10 μm. (b) Heatmap of CNIH1-4 expression across diverse human tissues based on bulk RNA-sequencing data from the GTex portal ( https://www.gtexportal.org/home/ ), using the Multi-Gene Query tool. Expression values are shown as Transcripts per Million (TPM). (c) Relative RNA expression levels of CNIH1-4 in a published dataset of 12 human nociceptor cell types ( https://sensoryomics.shinyapps.io/RNA-Data/ ), known for endogenous DOR expression. Expression values of CNIH1-4 shown in colors indicated in the figure, with 75th and 25th percentile of overall RNA expression levels within each cell type shown as lines across the graph.

Article Snippet: Primary antibody incubation was performed overnight at 4 °C in a humidified dark chamber with the following antibodies: CNIH1 (Sigma-Aldrich, HPA002544, 1:100), Giantin (Geneva Antibody Facility, ABCD_AA341, 1:100), and HA-Tag (6E2) (Cell Signaling Technology, 2367S, 1:200).

Techniques: Confocal Microscopy, Expressing, RNA Sequencing, RNA Expression